In the lab, Calle and her colleagues found that exposing Salmonella to the copper bead solution resulted in dramatic reductions in the population of the bacterial pathogen. Based on those results, they hoped that their animal experiments would show that the copper beads could reduce microbial pathogens in the gut, as well.
They divided a group of 48 6-month-old pigs—half female, half male—into two groups. During the second week of a 3-week study, the first group received normal feed, and the other received feed with added copper beads. Researchers collected feces from the pigs throughout the experiment, using atomic spectroscopy to measure copper levels and 16s RNA sequencing to identify microbial species.
Calle and her colleagues selected two families of bacteria to study as indicators of how the copper changed the gut microbiome. One was Enterobacteriaceae, a large collection of microbes that includes Salmonella, Shigella, Escherichia coli and others, including many that can be pathogenic. The other family included lactic acid bacteria, which have been linked to health or nutritional benefits.
Animals in the experimental group had about 20 times as much copper in their feces as animals in the control group, and after they stopped receiving copper their levels of the metal returned to baseline. Those measurements confirmed that the beads had effectively delivered the metal.
Analyses of the microbial populations showed that the copper additive led to higher levels of Enterobacteriaceae and lower levels of lactic acid bacteria in the control group—which wasn't the change the researchers had hoped to see.
Click here to see more...