Porcine Reproductive and Respiratory Syndrome (PRRS) virus was first detected in the U.S. in 1987. Pigs that contract the disease have extreme difficulty reproducing, don't gain weight and have a high mortality rate. To date, no vaccine has been effective, and the disease costs North American farmers more than $660 million annually. Now, a team of researchers from the University of Missouri, Kansas State University, and Genus plc have bred pigs that are not harmed by the disease.
"Once inside the pigs, PRRS needs some help to spread; it gets that help from a protein called CD163," said Randall Prather, distinguished professor of animal sciences in the College of Agriculture, Food and Natural Resources. "We were able to breed a litter of pigs that do not produce this protein, and as a result, the virus doesn't spread. When we exposed the pigs to PRRS, they did not get sick and continued to gain weight normally."
For years, scientists have been trying to determine how the virus infected pigs and how to stop it. Previously, researchers believed that the virus entered pigs by being inhaled into the lungs, where it attached to a protein known as sialoadhesin on the surface of white blood cells in the lungs.
However, two years ago Prather's group showed that elimination of sialoadhesin had no effect on susceptibility to PRRS. A second protein, called CD163, was thought to "uncoat" the virus and allow it to infect the pigs. In their current study, Prather's team worked to stop the pigs from producing CD163.
"We edited the gene that makes the CD163 protein so the pigs could no longer produce it," said Kristin Whitworth, co-author on the study and a research scientist in MU's Division of Animal Sciences. "We then infected these pigs and control pigs; the pigs without CD163 never got sick.
This discovery could have enormous implications for pig producers and the food industry throughout the world."
While the pigs that didn't produce CD163 didn't get sick, scientists also observed no other changes in their development compared to pigs that produce the protein.
The early-stage results of this research are promising. The University of Missouri has signed an exclusive global licensing deal for potential future commercialization of virus resistant pigs with the Genus, plc. If the development stage is successful, the commercial partner will seek any necessary approvals and registration from governments before a wide market release.
"The demonstration of genetic resistance to the PRRS virus by gene editing
These pigs were bred to be resistant to the PRRS virus. They were exposed to the virus with other pigs and never experienced symptoms of the disease.
is a potential game changer for the pork industry," said Jonathan Lightner, Chief Scientific Officer and Head of R&D of Genus plc. "There are several critical challenges ahead as we develop and commercialize this technology; however, the promise is clear, and Genus is committed to developing its potential. Genus is dedicated to the responsible exploration of new innovations that benefit the well-being of animals, farmers, and ultimately consumers."
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