Researchers Identify Genes Potentially Responsible for Sugarcane's Resistance to Pests, Cold and Drought

By Ricardo Muniz

A study conducted at the State University of Campinas (UNICAMP) in Brazil has identified orphan genes in wild sugarcane (Saccharum spontaneum), a species with exceptional resistance to biotic stresses such as nematodes, fungi, bacteria and other pests and diseases, and abiotic stresses such as cold, drought, salinity and nutritionally deficient soil.

According to an article on the study published in the journal Frontiers in Plant Science, the scientists responsible had set out to see if the orphan genes in S. spontaneum played a significant role in its stress resistance properties.

All living beings have genes that closely resemble those of other organisms' genomes. Plants, for example, share the genes involved in photosynthesis. On the other hand, most organisms also have orphan or lineage-specific genes.

Orphan genes are found in a particular taxonomic group with no significant sequence similarity to genes from other lineages. They are sometimes called taxonomically restricted genes for this reason.

Birds, for example, have some genes that differ a great deal from those of mammals. Recent research has shown that even organisms in closely related species belonging to the same genus can have genes not shared by other species.

The researchers were interested in S. spontaneum because of characteristics such as past whole-genome duplication events that resulted in several copies of the same gene. Scientific evidence suggests orphan genes can originate in copies of pre-existing genes whose sequences change over time owing to mutations and eventually differ entirely from the original sequences.

Another possible explanation for the origin of orphan genes could be reorganization of genomic regions that do not encode genes, frequently seen in organisms with complex genomes, such as sugarcane.

"In the study, we identified parts of the genome of S. spontaneum that have no similarities to genes in any other organism. We believe they may be responsible for physiological traits or properties peculiar to the species," said Cláudio Benício Cardoso-Silva, first author of the article. He conducted the project as postdoctoral research at UNICAMP's Center for Molecular Biology and Genetic Engineering (CBMEG).

"As these plants evolved, some genes were expressed to a greater or lesser extent in response to various types of abiotic stress, particularly cold. This may mean they're regulated as a result of these stresses," said Cardoso-Silva, whose postdoctoral research was supervised by Anete Pereira de Souza, professor of plant genetics at UNICAMP's Institute of Biology and last author of the article.

The researchers do not believe they can categorically conclude that the orphan genes they identified make the plant more stress-tolerant based on the results of the study. "But the fact that they're regulated under conditions of stress serves as an alert to the possibility that they may play an important role in these processes," he said.