New Technique Allows More Labs to Transform Maize Efficiently
Maize is one of the world’s most widely used crops. Its presence is felt in food, fuel, medicine, and pet products. However, efforts to bioengineer maize for modern agricultural needs have been limited by the technical difficulty of working with the crop.
A newly published study offers a breakthrough. Scientists from multiple institutions have improved a method that allows genetic transformation of maize using the central leaves, or leaf whorls, from seedlings. This avoids the need to harvest immature embryos from greenhouse-grown plants.
Traditional maize transformation relies on costly infrastructure and is limited to commercial labs. The new method allows seedlings to be used after just two weeks of growth, making research possible even in basic lab settings.
The study tested this method using two helper plasmids—one commercial and one publicly available. When applied to both common and difficult genotypes like B73, the results were highly effective.