The bill would make it an offence for a person to enter onto a livestock farm without permission.
Individuals who break the law could face a summary conviction of up to six months in jail and a fine up to $50,000.
Companies who disobey the law could face fines up to $500,000.
Barlow introduced a similar bill during the previous session of Parliament.
Bill C-205 received support from Conservative, NDP and Bloc MPs at its second reading.
Agriculture Minister Marie-Claude Bibeau was among those to vote against the bill.
Multiple ag groups also voiced support for the bill.
But Bill C-205 didn’t make the committee stage because of the summer 2021 election call.