If the hay has already been cut and it tests positive, Harty says the recommendation is to send a core sample in after the hay is baled so that a representative sample can be collected and sent to a lab for quantitative analysis prior to feeding.
A representative sample needs to be taken from different areas across the field to reflect topography and soil differences, as these factors can affect nitrate levels. Harty advises selecting plants from at least 10 different areas and cutting the plant at ground level or pulling it out of the ground so that the lowest growth nodes can be tested, as the highest concentration of nitrate is in the lowest third of the plant. The more representative the sample, the better the test will work to identify potential nitrate concerns, she says.
"Be diligent about testing forages prior to haying or grazing to prevent the negative impacts that nitrates can cause, specifically in ruminant animals," Harty says. "If crops, such as wheat, fail to make grain, and producers plan to utilize them for a hay crop, test them for nitrates prior to harvest to determine their safety level. The higher the level of nitrogen fertilizer that is applied, the higher the risk for nitrate toxicity. Always err on the side of caution and have feeds tested."
Source : sdstate.edu